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  Published Papers

Published 2006

  • Burden JP, Possee RD, Sait SM, King LA, Hails RS. (2006) Phenotypic and genotypic characterisation of persistent baculovirus infections in populations of the cabbage moth (Mamestra brassicae) within the British Isles. Arch Virol. Apr;151(4):635-49. Epub 2005 Nov 17.
  • Hitchman RB, Siaterli EA, Nixon CP, King LA. Quantitative real-time PCR for rapid and accurate titration of recombinant baculovirus particles. Biotechnol Bioeng. 2006 Sep 1; [Epub ahead of print]
  • Hitchman RB, Hodgson DJ, King LA, Hails RS, Cory JS & Possee RD Host mediated selection of pathogen genotypes as a mechanism for the maintenance of baculovirus diversity in the field. Journal for Invertebrate Pathology (TBP)
  • Kelly BJ, King LA, Possee RD, Chapple SD. (2006) Dual mutations in the Autographa californica nucleopolyhedrovirus FP-25 and p35 genes result in plasma-membrane blebbing in Trichoplusia ni cells. J Gen Virol. Mar;87(Pt 3):531-6.
  • Graham RI, Rao S, Possee RD, Sait SM, Mertens PP, Hails RS. (2006) Detection and characterisation of three novel species of reovirus (Reoviridae), isolated from geographically separate populations of the winter moth Operophtera brumata (Lepidoptera: Geometridae) on Orkney. J Invertebr Pathol. 2006 Feb;91(2):79-87. Epub Jan 18.
  • Hitchman RB, Siaterli EA, Nixon CP, King LA. (2006) Quantitative real-time PCR for rapid and accurate titration of recombinant baculovirus particles. Biotechnol Bioeng. Sep 1; [Epub ahead of print]

Published 2004

  • Graham RI, Tyne WI, Possee RD, Sait SM, Hails RS. (2004) Genetically variable nucleopolyhedroviruses isolated from spatially separate populations of the winter moth Operophtera brumata (Lepidoptera: Geometridae) in Orkney. J Invertebr Pathol. Sep;87(1):29-38.
  • King LA, Hitchman R, Possee RD (2005) Recombinant Baculovirus Isolation In: Baculovirus Expression Protocols Chapter 3. (Ed. by D. Murhammer) Humana Press Inc.
  • Hodgson DJ, Hitchman RB, Vanbergen AJ, Hails RS, Possee RD, Cory JS. (2004) Host ecology determines the relative fitness of virus genotypes in mixed-genotype nucleopolyhedrovirus infections. J Evol Biol. Sep;17(5):1018-25.
  • Saville GP, Patmanidi AL, Possee RD, King LA. (2004) Deletion of the Autographa californica nucleopolyhedrovirus chitinase KDEL motif and in vitro and in vivo analysis of the modified virus. J Gen Virol. Apr;85(Pt 4):821-31.

Published 2003

  • Patmanidi AL, Possee RD, King LA. (2003) Formation of P10 tubular structures during AcMNPV infection depends on the integrity of host-cell microtubules. Virology. Dec 20;317(2):308-20.
  • Hodgson DJ, Hitchman RB, Vanbergen AJ, Hails RS, Possee RD, Cory JS. Host ecology determines the relative fitness of virus genotypes in mixed-genotype nucleopolyhedrovirus infections. J Evol Biol. 2004 Sep;17(5):1018-25.

Published 2002

  • Saville GP, Thomas CJ, Possee RD, King LA. (2002). Partial redistribution of the Autographa californica nucleopolyhedrovirus chitinase in virus-infected cells accompanies mutation of the carboxy-terminal KDEL ER-retention motif. J Gen Virol. Mar;83(Pt 3):685-94.
  • Hodgson DJ, Hitchman RB, Vanbergen AJ, Hails RS, Hartley SE, Possee RD, Watt, AD & Cory, JS. Part 3: Chapter 15, Host-pathogen interactions. The existence and persistence of genotypic variation in nucleopolyhedrovirus populations. In Genes in the Environment, Eds: RS Hails, JE Beringer & HCJ Godfray. The 15th Special Symposium of the British Ecological Society, St Catherines College, Oxford 17-19 September 2001. British Ecological Society & Blackwell Science Ltd.

Published 2000

  • King, LA and Possee, RD. (2000). Insect cell culture for baculovirus replication. Encyclopaedia of Life Sciences, Macmillan Press London. In press.
  • Possee,RD and King, LA. (2000). Baculoviruses Encyclopaedia of Life Sciences. Macmillan Press, London. In press.
  • Thomas, CA, Gooday, GW, King, LA and Possee, RD. (2000). Mutagenesis of the active site coding region of the Autographa californica nucleopolyhedrovirus chitinase gene. J. Gen. Virol. 81, 1403-1411.
  • Grasela, JJ., Mcintosh, AH., Goodman, CL., Wilson, LE And King, LA. (2000). Expression of the green fluorescent protein carried by Autographa californica multiple polyhedrovirus in insect cells. In Vitro Cell. Dev. Biol.- Animal. 36, 205-210.
  • Shi, J., Thomas, CJ., King, LA., Hawes, CR., Possee, RD., Edwards, ML, Pallett, D and Cooper, JI. (2000). The expression in flue-cured and in oriental tobacco cultivars of a baculovirus-derived chitinase gene. Annals Applied Biology. 136, 1-8.

Published 1999


  • Possee, RD., Thomas, CA and King, LA. (1999). The use of baculovirus vectors for the production of membrane proteins in insect cells. Boichem. Soc. Trans. 27, 928-932.
  • King, LA, Thomas, CJ, Wilkinson, N and Possee, RD. (1999). Baculovirus expression of green fluorescent protein using baculovirus vectors. Methods in Enzymology (Ed. P.M. Conn). 302, pp 394-408.
  • Griffiths, CM, Barnett, AL, Ayres, MD, Windass, J, King, LA and Possee, RD. (1999). In vitro host range of AcMNPV recombinants lacking functional p35, iap1 or iap2. J. Gen. Virol., 80, 1055-1066.
  • Merrington, C, King, LA and Possee, RD. (1999). Baculovirus expression systems. In. Protein expression - a practical approach. Vol I (Eds. Higgins, SJ and Hames, BD). Oxford University Press, pp 101-127.

Published 1998

  • King, LA, Miller, DP, Wilkinson, N and Marlow, SA. (1998). Entomopoxviruses. In: The Insect Viruses. (Eds. L.K. Miller & A. Ball). Plenum Press, pp 1-29.
  • Thomas, CA, Hawes, CR, Lee, BY, Min, M-K, King, LA and Possee, RD. (1998). Localisation of a baculovirus-induced chitinase in the insect cell endoplasmic reticulum. Journal of Virology, 72, 10207-10212.
  • Marlow, SA, Wilson, LE, Lawrie, AM, Wilkinson, N and King, LA. (1998). Assembly of Amsacta moorei entomopoxvirus spheroidin into spheroids following synthesis in recombinant baculovirus-infected cells. J. Gen.Virol., 79, 623-628.
  • King, LA, Marlow, SA, Obosi, LA and Possee, RD. (1998). The baculovirus Expression Vector System II: Amplification and Characterization of Recombinant Viruses. In Cell Biology Handbook 2nd Edn. (Ed. J. Celis), Academic Press, pp.211-217.
  • King, LA, Marlow, SA, Wilson, LE and Possee, RD. (1998). The Baculovirus Expression Vector systems I: Production and Isolation of Recombinant Virus. In Cell Biology Handbook 2nd Edn. (Ed. J. Celis), Academic Press, pp. 204-210.

Published 1997

  • Hawtin, RE, Thomas, CA, Gooday, GW, Kuzio, JA, King, LA and Possee, RD. (1997). Liquefaction of Autographa californica nucleopolyhedrovirus-infected cells is dependent on the integrity of virus-encoded chitinase and cathepsin genes. Virology, 238, 243-253.
  • Possee, RD, Barnett, AL, Hawtin, RE and King, LA (1997). Engineered viruses for pest control. Pesticide Science, 51, 462-470.
    McCarroll, L and King, LA. (1997). Stable insect cell cultures for recombinant protein production. Current Opinion in Biotechnology 8, 590-594.
  • Obosi, LA, Beadle, DJ, Hen, R, Bermudez, I and King, LA. (1997). Mutational analysis of the mouse 5-HT7 receptor; importance of the third intracellular loop for receptor-G-protein coupling. FEBS Letters 412, 321-324.
  • Watkins, MJ, Kiyatkin, N, Hughes, D, Beadle, DJ and King, LA (1997). Study on the biological properties of a novel recombinant baculovirus. BCPC Monograph, Microbial Insecticides: Novelty or Necessity? 68, 279-284.
  • Saville, GP, Hughes, DS, Shreeve, T, Possee, RD and King, LA (1997). Baculovirus insecticides: detection of latent baculoviruses in natural insect populations. BCPC Monograph, Microbial Insecticides: Novelty or Necessity? 68, 255-260.
  • King, LA and Possee, RD (1997). A future for baculovirus insecticides ? Focus on Biopesticides Plus (January), pp. 1-2. The Royal Society of Chemistry.
  • Aspinwall, LS, Bermudez, I, King, LA and Wafford, KA. (1997). The interactions of hexachlorocyclohexane isomers with human GABA-A receptors expressed in Xenopus oocytes. Journal of Pharmacology and Experimental Therapeutics 282, 1557-1564.
  • Wilson, LE, Wilkinson, N, Marlow, SA, Possee, RD and King, LA. (1997). Identification of recombinant baculoviruses using green fluorescent protein as a selectable marker. Biotechniques 22, 674-681.
  • Hughes, DS, Possee, RD and King, LA. (1997). Detection of transcriptional factors in insect cells harbouring a persistent Mamestra brassicae nuclear polyhedrosis virus infection. J. Gen. Virology 78, 1801-1805.

Published 1996

  • Obosi, LA, Schuette, DG, Europe-Finner, N, Hen, R, King, LA and Bermudez, I. (1996). Characterization of the Drosophila 5HT-dro1 and 5HT-dro2B serotonin receptors using baculovirus vectors. FEBS Letters 381, 233-236.
  • Atkinson, AE, Henderson, J, Hawes, CR and King, LA. (1996). Efficient membrane targeting of the chick nicotinic acetylcholine receptor a-subunit in insect cell lines. Cytotechnology, 19, 37-42.
  • Merrington, CL, Kitts, PA, King, LA and Possee, RD. (1996). Autographa californica nucleopolyhedrosis lef2 mutant: consequences for DNA replication and very late gene expression. Virology, 217, 338-348.

Published 1995

  • Henderson, J, Napier, RM, Lazurus, CM and King, LA (1995). Transgene expression in insect cells: comparison of transformation and baculovirus infection. Molecular Biology of the Cell 7, (No.SS), 448.
  • Henderson, J, Atkinson, AE, Lazarus, CM, Hawes, CR, Napier, RM, McDonald, H and King, LA. (1995). Stable expression of the maize auxin-binding protein in continuously expressing insect cell lines. FEBS, 171, 293-296.
  • Amar, M, Smith, LA, Needham, M, Beadle, DJ, King, LA and Bermudez, I. (1995). Expression of a bovine GABAA Receptor a-1subunit cDNA in murine erythroleukaemia cells. J. of Receptor and Signal Transduction Research. 15, 71-80.
  • Smith, LA, Amar, M, Harvey, RJ, Darlison, MG, Earley, FGP, Beadle, DJ, King, LA and Bermudez, I. (1995). The production of a stably transformed insect cell line expressing an invertebrate GABAA receptor ß-subunit. J. of Receptor and Signal Transduction Research. 15, 33-42.
  • Kiyatkin, N, Kulikovskaya, I, Grishin, EV, Beadle, DJ and King, LA. (1995). Functional characterization of black widow spider neurotoxins synthesised in insect cells. Eur. J. Biochem. 230, 854-859.
  • Hawtin, RE, Arnold, K, Ayres, MA, Gooday, GW, Chappell, LH, Kitts, PA, King, LA and Possee, RD. (1995). Identification and preliminary characterization of a chitinase gene in the Autographa californica nuclear polyhedrosis virus genome. Virology, 212, 673-685.
  • Palmer, CP, Miller, D, Marlow, SA, Wilson, LE, Lawrie, AM and King, LA. (1995). Genetic modification of an entomopoxvirus; deletion of the spheroidin gene does not affect virus replication in vitro. Journal of General Virology 76 15-23.
  • Lawrie, AM, Ogden, JE and King, LA. (1995). High level synthesis and secretion of human urokinase using a late gene promoter of the Autographa californica nuclear polyhedrosis virus. Biotechnology 39, 1-8.

Published 1994

  • Hughes, DS, Possee, RD and King, LA. (1994). Quantitation of latent Mammestra brassicae nuclear polyhedrosis virus in M. brassicae insects using a PCR-scintillation proximity assay. Journal of Virological Methods, 50 21-28.
  • Possee, RD and King, LA. (1994). Molecular approaches to the design of biotic crop protection agents. In: Molecular Biology in Crop Protection (eds, Marshall, G and Walters, DR) pp. 68-97, Chapman and Hall, London.
  • King, LA, Possee, RD, Hughes, DS, Marlow, SA, Palmer, CP, Miller, DP, Atkinson, AE, Lawrie, AM, Pickering, J, Joyce, KA and Beadle, DJ. (1994). Advances in insect virology. In. Advances in Insect Physiology. 25, 1-73, Academic Press, London.
  • King, LA, Mann, SG, Lawrie, AM and Possee, RD. (1994). Production and isolation of recombinant baculoviruses. In. Cell Biology Handbook (ed, Celis, K), pp. 148-154, Academic Press, New York.

Published 1993

  • Sgard, F, Obosi, LA, King, LA and Windass, JD. (1993). ALS and SAD-like nicotinic acetylcholine receptor subunit genes are widely distributed in insects. Insect Molecular Biology 2, 215-223.
  • Joyce, KA, Atkinson, AE, Bermudez, I, Beadle, DJ and King, LA. (1993). Synthesis of functional GABA-A receptors in stable insect cell lines. FEBS Letters 335, 61-64.
  • Marlow, SA, Billam, LJ, Palmer, CP and King, LA. (1993). Replication and morphogenesis of Amsacta moorei entomopoxvirus in cultured Estigmene area cells. Journal of General Virology 74, 1457-1461.
  • Hughes, DS, Possee, RD and King, LA. (1993). Activation and detection of a latent baculovirus resembling Mamestra brassicae nuclear polyhedrosis virus in M. brassicae larvae. Virology 194, 608-615.

Published 1992

  • King, LA, Mann, SG, Marlow, SA, Bermudez, I, Lawrie, AM, Obosi, LA and Atkinson, AE. (1992). Alternative insect cell lines for baculovirus expression vectors. In. (eds., Vlak, J., Schlaeger, E. and Bernard, A) Baculovirus and recombinant protein production processes. pp. 157-165. Editiones Roche, Basel.
  • Marlow, SA, Palmer, CP and King, LA. (1992). Cytopathic effects of Amsacta moorei entompoxvirus infection on the cytoskeleton of Estigmene acrea cells. Virus Research 26, 41-55.
  • Atkinson, AE, Bermudez, I, Darlison, MG, Barnard, EA, Earley, FPG, Possee, RD, Beadle, DJ and King, LA. (1991). Assembly of functional GABAA receptors in insect cells using baculovirus vectors. Neuroreport 3, 597-600.
  • Weitzman, MD, Possee, RD and King, LA. (1992). Genetic analysis of two strains of Panolis flammea nuclear polyhedrosis virus. Journal of General Virology 73, 1881-1886.
  • King, LA and Possee, RD. (1992). The baculovirus expression vector system: a laboratory guide. Chapman and Hall, London.
  • Possee, RD, King, LA, Hughes, DS, Weitzman, MD, Mann, SG, and Bishop, DHL. (1992). Progress in the genetic modification and field-release of baculovirus insecticides. In. The release of genetically modified microorganisms - REGEM2 (Eds, Stewart-Tull, DES and Sussman, M), pp. 47-58, Plenum Press, New York and London.
  • Hawtin, RE, King, LA and Possee, RD. (1992). The prospects for genetically engineered baculovirus insecticides. Pesticide Science, 34, 9-15.

Published 1991

  • Atkinson, AE, Bermudez, I, Beadle, DJ and King, LA. (1991). Expression of neurotransmitter receptors in insect cells using baculovirus vectors. Pesticide Science, 33
  • King, LA, Atkinson, AE, Obosi, LA, Bermudez, I and Beadle, DJ. (1991). Expression of neurotransmitter receptors in insect cells using baculovirus vectors. In. Neurotox '91 (Ed: Duce, I.R.), Elsevier Press, Amsterdam. pp. 191-202, Elsevier, Amsterdam.
  • King, LA, Kaur, K, Mann, SG, Lawrie, AM, Steven, J and Ogden, J. (1991). Secretion of single chain urokinase-type plasminogen activator from insect cells. Gene, 106, 151-157.
  • King, LA, Mann, SG, Mulshaw, SH and Lawrie, AM. (1991). Comparison of Autographa californica nuclear polyhedrosis virus replication in two insect cell lines, Spodoptera frugiperda and Mamestra brassicae. Virus Research, 19, 93-104.

Published 1990

  • Atkinson, AE, Earley, FPG, Beadle, DJ and King, LA. (1990). Expression and characterization of the chick nicotinic acetylcholine receptor alpha-subunit in insect cells using a baculovirus vector. European Journal of Biochemistry, 192, 451-458.
  • Weitzman, MD, Possee, RD and King, LA. (1990). Virus Insecticides: genetic analysis of Panolis flammea nuclear polyhedrosis virus. In: Pesticides and Alternatives (Ed: Casida, JE) Elsevier Press, Amsterdam, pp. 123-130.
  • Possee, RD, Weyer, U and King, LA. (1990). Recombinant antigen production using baculovirus expression vectors. In: Control of Virus Disease (Ed: Griffiths, P, Dimmock, N and Madeley, C R). Society for General Microbiology Symposium Volume 45. pp 53-76. Cambridge University Press.
  • Atkinson, AE, Weitzman, MD, Obosi, L, Beadle, DJ and King, LA. (1990). Expression of foreign genes in insect cells using baculovirus vectors. Pesticide Science, 28, 215-224.
  • King, LA, Atkinson, AE, Mann, SG, Lawrie, AM, Mulshaw, SH, Obosi, LA, Beadle, DJ and Earley, F. (1990). Expression and characterization of the chick nicotinic acetylcholine receptor alpha-subunit and E. coli beta-galactosidase in two insect cell lines using baculovirus vectors. In: Molecular Insect Science (Hagedorn, HH, Hildebrand, JG, Kidwell, MG and Law, JH, Eds) p. 107. Plenum Press, New York.
  • Atkinson, AE, Obosi, LA, Beadle, DJ and King, LA. (1989). Expression of the chick nicotinic acetylcholine receptor alpha-subunit in insect cells using a baculovirus vector. In: Ion Transport (Keeling, D and Benham, C, Eds) p.367. Academic Press, London.
  • Mann, SG and King, LA. (1989). Efficient uptake of baculovirus DNA into insect cells by electroporation. Journal of General Virology, 70, 5301-5305.


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